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product summary
company name :
Addgene
product type :
cDNA
product name :
pFGET19_Ulp1
catalog :
64697
citations: 23
Reference
Singh A, Kr x119 x17c el A. Calcium-assisted sortase A cleavage of SUMOylated metallothionein constructs leads to high-yield production of human MT3. Microb Cell Fact. 2023;22:125 pubmed publisher
Mani S, Kaur A, Jakhar K, Kumari G, Sonar S, Kumar A, et al. Targeting DPP4-RBD interactions by sitagliptin and linagliptin delivers a potential host-directed therapy against pan-SARS-CoV-2 infections. Int J Biol Macromol. 2023;245:125444 pubmed publisher
Demay F, Hallier M, Georgeault S, Com E, Cattoir V, Goude R, et al. Simple purification and characterization of soluble and homogenous ABC-F translation factors from Enterococcus faecium. Protein Expr Purif. 2023;210:106325 pubmed publisher
Hamaker N, Lee K. High-efficiency and multilocus targeted integration in CHO cells using CRISPR-mediated donor nicking and DNA repair inhibitors. Biotechnol Bioeng. 2023;: pubmed publisher
Martinez D Alto A, Yan X, Detomasi T, Sayler R, THOMAS W, Talbot N, et al. Characterization of a unique polysaccharide monooxygenase from the plant pathogen Magnaporthe oryzae. Proc Natl Acad Sci U S A. 2023;120:e2215426120 pubmed publisher
Meeks K, Tanner J. Expression and kinetic characterization of PYCR3. Arch Biochem Biophys. 2023;733:109468 pubmed publisher
Chen Z, Huang J, Li J. Protein purification, crystallization, and structure determination of human DEAD-box RNA helicase DDX21 in different unwinding states. STAR Protoc. 2022;3:101642 pubmed publisher
Kozielski F, Sele C, Talibov V, Lou J, Dong D, Wang Q, et al. Identification of fragments binding to SARS-CoV-2 nsp10 reveals ligand-binding sites in conserved interfaces between nsp10 and nsp14/nsp16. RSC Chem Biol. 2022;3:44-55 pubmed publisher
Cáceres J, Bailey C, Yokoyama K, Greene B. Selenocysteine substitutions in thiyl radical enzymes. Methods Enzymol. 2022;662:119-141 pubmed publisher
Merritt C, Chun E, Fattah R, Silva L, Ma Q, Moayeri M, et al. Imaging of anthrax intoxication in mice reveals shared and individual functions of surface receptors CMG-2 and TEM-8 in cellular toxin entry. J Biol Chem. 2021;298:101467 pubmed publisher
Birtles D, Lee J. Identifying Distinct Structural Features of the SARS-CoV-2 Spike Protein Fusion Domain Essential for Membrane Interaction. Biochemistry. 2021;60:2978-2986 pubmed publisher
Fiore K, Phan H, Robkis D, Walters C, Petersson E. Incorporating thioamides into proteins by native chemical ligation. Methods Enzymol. 2021;656:295-339 pubmed publisher
Canal B, McClure A, Curran J, Wu M, Ulferts R, Weissmann F, et al. Identifying SARS-CoV-2 antiviral compounds by screening for small molecule inhibitors of nsp14/nsp10 exoribonuclease. Biochem J. 2021;478:2445-2464 pubmed publisher
Liu J, Xue Z, Vann K, Shi X, Kutateladze T. Protocol for Biochemical Analysis and Structure Determination of the ZZ Domain of the E3 Ubiquitin Ligase HERC2. STAR Protoc. 2020;1:100155 pubmed publisher
Patchsung M, Jantarug K, Pattama A, Aphicho K, Suraritdechachai S, Meesawat P, et al. Clinical validation of a Cas13-based assay for the detection of SARS-CoV-2 RNA. Nat Biomed Eng. 2020;4:1140-1149 pubmed publisher
Yoganand K, Muralidharan M, Nimkar S, Anand B. Fidelity of prespacer capture and processing is governed by the PAM-mediated interactions of Cas1-2 adaptation complex in CRISPR-Cas type I-E system. J Biol Chem. 2019;294:20039-20053 pubmed publisher
Linova M, Risør M, Jørgensen S, Mansour Z, Kaya J, Sigurdarson J, et al. A novel approach for production of an active N-terminally truncated Ulp1 (SUMO protease 1) catalytic domain from Escherichia coli inclusion bodies. Protein Expr Purif. 2020;166:105507 pubmed publisher
de Araujo E, Erdogan F, Neubauer H, Meneksedag Erol D, Manaswiyoungkul P, Eram M, et al. Structural and functional consequences of the STAT5BN642H driver mutation. Nat Commun. 2019;10:2517 pubmed publisher
Winton A, Baptiste J, Allen M. A versatile expression vector for the growth and amplification of unmodified phage display polypeptides. Protein Expr Purif. 2018;149:31-36 pubmed publisher
de Araujo E, Manaswiyoungkul P, Israelian J, Park J, Yuen K, Farhangi S, et al. High-throughput thermofluor-based assays for inhibitor screening of STAT SH2 domains. J Pharm Biomed Anal. 2017;143:159-167 pubmed publisher
Baranova N, Loose M. Single-molecule measurements to study polymerization dynamics of FtsZ-FtsA copolymers. Methods Cell Biol. 2017;137:355-370 pubmed publisher
de Araujo E, Geletu M, Gunning P. Strategies for over-expression and purification of recombinant full length STAT5B in Escherichia coli. Protein Expr Purif. 2017;129:1-8 pubmed publisher
Guerrero F, Ciragan A, Iwaï H. Tandem SUMO fusion vectors for improving soluble protein expression and purification. Protein Expr Purif. 2015;116:42-9 pubmed publisher
product information
Catalog Number :
64697
Product Name :
pFGET19_Ulp1
article :
doi10.1016/j.pep.2015.08.019
id15787
pubmed_id26297996
bacterial resistance :
Kanamycin
cloning :
backbonepET-28b(+)
backbone_mutation
backbone_originNovagen
backbone_size5368
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
H6-Ulp1 from pHYRS52 into pET-28b(+)
origin :
37
pi :
alt_names
SUMO protease ULP1
NIB1
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5XbaI
promoterT7
sequencing_primer_3T7 term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYPL020C, NIB1
geneULP1
id856087
genbank_ids
YPL020CNIB1
http://www.ncbi.nlm.nih.gov/gene/856087
mutationUlp1(403-621)
nameULP1
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
32644
Other
Saccharomyces cerevisiae
tags
locationN terminal on backbone
tag6xHis
resistance markers :
221
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA