This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
HRas (G12V, E37G)-pcw107-V5
catalog :
64640
citations: 1
Reference
Martz C, Ottina K, Singleton K, Jasper J, Wardell S, Peraza Penton A, et al. Systematic identification of signaling pathways with potential to confer anticancer drug resistance. Sci Signal. 2014;7:ra121 pubmed publisher
product information
Catalog Number :
64640
Product Name :
HRas (G12V, E37G)-pcw107-V5
article :
doi10.1126/scisignal.aaa1877
id9615
pubmed_id25538079
bacterial resistance :
Ampicillin
cloning :
backbonepcw107 (V5)
backbone_mutation
backbone_originJohn Doench/Kathleen Ottina
backbone_size9240
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth notes :
TTCC (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-S1-V5
growth strain :
when used to produce lentivirus, express physiological levels of insert
origin :
37
pi :
alt_names
NM_001130442.1
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterPGK
sequencing_primer_3WPRE-R
sequencing_primer_5hPGK-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras
geneHRAS
id3265
genbank_ids
mutationG12V, E37G
nameHRAS (transcript variant 3)
shRNA_sequence
size570
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagV5
plasmid copy :
Scott Lowe (Addgene plasmid # 18745)
resistance markers :
432
2311
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA