p53 (dominant negative R175H mutant)-pcw107-V5 | Addgene 64638 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

p53 (dominant negative R175H mutant)-pcw107-V5

catalog :

64638

citations: 2

Reference
Przanowski P, Przanowska R, Guertin M. ANKLE1 cleaves mitochondrial DNA and contributes to cancer risk by promoting apoptosis resistance and metabolic dysregulation. Commun Biol. 2023;6:231 pubmed publisher
Martz C, Ottina K, Singleton K, Jasper J, Wardell S, Peraza Penton A, et al. Systematic identification of signaling pathways with potential to confer anticancer drug resistance. Sci Signal. 2014;7:ra121 pubmed publisher

product information

Catalog Number :

64638

Product Name :

p53 (dominant negative R175H mutant)-pcw107-V5

article :

doi	10.1126/scisignal.aaa1877
id	9615
pubmed_id	25538079

bacterial resistance :

Ampicillin

cloning :

backbone

pcw107 (V5)

backbone_mutation

backbone_origin

John Doench/Kathleen Ottina

backbone_size

9240

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth notes :

TGCA (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-R1-V5

growth strain :

3rd generation lentiviral transfer plasmid. When used to produce lentivirus, express physiological levels of insert

origin :

pi :

alt_names

NM_001126114.2

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	PGK
sequencing_primer_3	WPRE-R
sequencing_primer_5	hPGK-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BCC7, BMFS5, LFS1, P53, TRP53
gene	TP53
id	7157

genbank_ids

mutation

R175H

name

TP53 (transcript variant 3)

shRNA_sequence

size

1026

species

9606

Homo sapiens