AR-V7-pcw107 | Addgene 64635 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

AR-V7-pcw107

catalog :

64635

citations: 2

Reference
Makhov P, Fazliyeva R, Tufano A, Uzzo R, Cai K, Serebriiskii I, et al. Acetyl-CoA Counteracts the Inhibitory Effect of Antiandrogens on Androgen Receptor Signaling in Prostate Cancer Cells. Cancers (Basel). 2022;14: pubmed publisher
Martz C, Ottina K, Singleton K, Jasper J, Wardell S, Peraza Penton A, et al. Systematic identification of signaling pathways with potential to confer anticancer drug resistance. Sci Signal. 2014;7:ra121 pubmed publisher

product information

Catalog Number :

64635

Product Name :

AR-V7-pcw107

article :

doi	10.1126/scisignal.aaa1877
id	9615
pubmed_id	25538079

bacterial resistance :

Ampicillin

cloning :

backbone

pcw107 (V5)

backbone_mutation

backbone_origin

John Doench/Kathleen Ottina

backbone_size

9240

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Lentiviral

growth notes :

GCTG (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-P1-STOP *Addgene sequence shows that there is an L57Q mutation in AR-V7 relative to NCBI sequences. There is also a QQQ insertion and a GGGG deletion relative to NM_000044.3. These mutations were in the original Sawyers lab clone and are not known to affect function.

growth strain :

when used to produce lentivirus, express physiological levels of insert

origin :

pi :

alt_names

NM_000044.3

cloning

clone_method	Gateway Cloning
cloning_site_3
cloning_site_5
promoter	PGK
sequencing_primer_3	WPRE-R
sequencing_primer_5	hPGK-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AIS, AR8, DHTR, HUMARA, HYSP1, KD, NR3C4, SBMA, SMAX1, TFM
gene	AR
id	367

genbank_ids

mutation

splice isoform*

name

AR (transcript variant 1, splice isoform)

shRNA_sequence

size

1932

species

9606

Homo sapiens