This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
JAK2 (V617F)-pcw107-V5
catalog :
64610
citations: 5
Reference
Yang J, Weisberg E, Liu X, Magin R, Chan W, Hu B, et al. Small molecule inhibition of deubiquitinating enzyme JOSD1 as a novel targeted therapy for leukemias with mutant JAK2. Leukemia. 2021;: pubmed publisher
Wang W, Tang Y, Xiao Q, Lee W, Cheng B, Niu Z, et al. Stromal induction of BRD4 phosphorylation Results in Chromatin Remodeling and BET inhibitor Resistance in Colorectal Cancer. Nat Commun. 2021;12:4441 pubmed publisher
Arenas E, Martínez Sabadell A, Rius Ruiz I, Román Alonso M, Escorihuela M, Luque A, et al. Acquired cancer cell resistance to T cell bispecific antibodies and CAR T targeting HER2 through JAK2 down-modulation. Nat Commun. 2021;12:1237 pubmed publisher
Umezawa Y, Akiyama H, Okada K, Ishida S, Nogami A, Oshikawa G, et al. Molecular mechanisms for enhancement of stromal cell-derived factor 1-induced chemotaxis by platelet endothelial cell adhesion molecule 1 (PECAM-1). J Biol Chem. 2017;292:19639-19655 pubmed publisher
Martz C, Ottina K, Singleton K, Jasper J, Wardell S, Peraza Penton A, et al. Systematic identification of signaling pathways with potential to confer anticancer drug resistance. Sci Signal. 2014;7:ra121 pubmed publisher
product information
Catalog Number :
64610
Product Name :
JAK2 (V617F)-pcw107-V5
article :
doi10.1126/scisignal.aaa1877
id9615
pubmed_id25538079
bacterial resistance :
Ampicillin
cloning :
backbonepcw107 (V5)
backbone_mutation
backbone_originJohn Doench/Kathleen Ottina
backbone_size9240
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth notes :
TGGT (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-D1-V5
growth strain :
when used to produce lentivirus, express physiological levels of insert
origin :
37
pi :
alt_names
NM_004972.3
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterPGK
sequencing_primer_3WPRE-R
sequencing_primer_5hPGK-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesJTK10
geneJAK2
id3717
genbank_ids
mutationV617F
nameJAK2
shRNA_sequence
size3399
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagV5
plasmid copy :
William Vainchecker Lab, Institut Gustave Roussy
resistance markers :
432
2311
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA