This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
FLAG-Rheb (Q64L)-pcw107
catalog :
64607
citations: 3
Reference
Yildirim A, Mozaffari Jovin S, Wallisch A, Schafer J, Ludwig S, Urlaub H, et al. SANS (USH1G) regulates pre-mRNA splicing by mediating the intra-nuclear transfer of tri-snRNP complexes. Nucleic Acids Res. 2021;49:5845-5866 pubmed publisher
Hu H, Shiflett L, Kobayashi M, Chao M, Wilson A, Mohr I, et al. TOP2β-Dependent Nuclear DNA Damage Shapes Extracellular Growth Factor Responses via Dynamic AKT Phosphorylation to Control Virus Latency. Mol Cell. 2019;74:466-480.e4 pubmed publisher
Martz C, Ottina K, Singleton K, Jasper J, Wardell S, Peraza Penton A, et al. Systematic identification of signaling pathways with potential to confer anticancer drug resistance. Sci Signal. 2014;7:ra121 pubmed publisher
product information
Catalog Number :
64607
Product Name :
FLAG-Rheb (Q64L)-pcw107
article :
doi10.1126/scisignal.aaa1877
id9615
pubmed_id25538079
bacterial resistance :
Ampicillin
cloning :
backbonepcw107 (V5)
backbone_mutation
backbone_originJohn Doench/Kathleen Ottina
backbone_size9240
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Lentiviral
growth notes :
AATG (N-term barcode, 17 nt upstream of CDS start). Working name: CTK-B3-STOP
growth strain :
when used to produce lentivirus, express physiological levels of insert
origin :
37
pi :
alt_names
cloning
clone_methodGateway Cloning
cloning_site_3
cloning_site_5
promoterPGK
sequencing_primer_3WPRE-R
sequencing_primer_5hPGK-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesRHEB2
geneRHEB
id6009
genbank_ids
NM_005614.3
mutationQ64L
nameRHEB
shRNA_sequence
size585
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFLAG
plasmid copy :
Peter Vogt (Addgene plasmid # 21050)
resistance markers :
432
2311
tags :
Unknown
terms :
Puromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA