pSF4 CMV 5TOP intron renilla TRICK CTE polyA | Addgene 64544 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pSF4 CMV 5TOP intron renilla TRICK CTE polyA

catalog :

64544

citations: 2

Reference
Voigt F, Eglinger J, Chao J. Detection of the First Round of Translation: The TRICK Assay. Methods Mol Biol. 2018;1649:373-384 pubmed publisher
Halstead J, Lionnet T, Wilbertz J, Wippich F, Ephrussi A, Singer R, et al. Translation. An RNA biosensor for imaging the first round of translation from single cells to living animals. Science. 2015;347:1367-671 pubmed publisher

product information

Catalog Number :

64544

Product Name :

pSF4 CMV 5TOP intron renilla TRICK CTE polyA

article :

doi	10.1126/science.aaa3380
id	10022
pubmed_id	25792328

bacterial resistance :

Ampicillin

cloning :

backbone

pSF4

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

5 TOP TRICK reporter mRNA

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	Tetracycline-inducible promoter and 5 UTR of human RPL32 from rpL32- -globin
sequencing_primer_3
sequencing_primer_5	TRE-F
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

12x PP7 stem-loops

shRNA_sequence

size

species

100

Synthetic