This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGL2-HKII-D
catalog :
64516
citations: 1
Reference
Mathupala S, Rempel A, Pedersen P. Glucose catabolism in cancer cells: identification and characterization of a marked activation response of the type II hexokinase gene to hypoxic conditions. J Biol Chem. 2001;276:43407-12 pubmed
product information
Catalog Number :
64516
Product Name :
pGL2-HKII-D
article :
doi10.1074/jbc.M108181200
id10021
pubmed_id11557773
bacterial resistance :
Ampicillin
cloning :
backbonepGL2-Basic
backbone_mutationNone
backbone_originPromega
backbone_size5598
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Luciferase
growth notes :
For stability the plasmid is maintained in E. coli SURE. For reporter studies it is propagated in E. coli SCS110 or other methylation deficient strain. Note: Addgene's quality control sequencing has identified a 24 base pair gap, compared to the depositor's reference sequence. However, the plasmid accurately reflects the construct as it was used in the associated publication, and should function as described.
growth strain :
AS-30D hepatoma hexokinase II 1.0 kbp promoter-luciferase reporter vector
origin :
37
pi :
alt_names
HKII
HK2
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5XbaI
promoterHexokinase II promoter
sequencing_primer_3GL primer 2
sequencing_primer_5GL primer 1
site_3_destroyed
site_5_destroyed1
entrez_gene
aliases
geneHk2
id25059
genbank_ids
U19605.2
mutationIsolated from AS-30D rat hepatoma
nameType II hexokinase gene, partial cds and promoter region
shRNA_sequence
size1487
species
10116
Rattus norvegicus
tags
location
tagfirefly luciferase
resistance markers :
2310
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA