This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
PTEF1-yEGFPCLN2PEST-pRS406
catalog :
64406
citations: 3
Reference
Young T, Liu P, Urbonaite G, Acar M. Quantitative Insights into Age-Associated DNA-Repair Inefficiency in Single Cells. Cell Rep. 2019;28:2220-2230.e7 pubmed publisher
Sarnoski E, Song R, Ertekin E, Koonce N, Acar M. Fundamental Characteristics of Single-Cell Aging in Diploid Yeast. iScience. 2018;7:96-109 pubmed publisher
Williams T, Espinosa M, Nielsen L, Vickers C. Dynamic regulation of gene expression using sucrose responsive promoters and RNA interference in Saccharomyces cerevisiae. Microb Cell Fact. 2015;14:43 pubmed publisher
product information
Catalog Number :
64406
Product Name :
PTEF1-yEGFPCLN2PEST-pRS406
article :
doi10.1186/s12934-015-0223-7 [pii]
id18620
pubmed_id25886317
bacterial resistance :
Ampicillin
cloning :
backboneGFPCLN2PEST-pRS406
backbone_mutationdestabilized GFP
backbone_originMateus and Avery 2000
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth strain :
TEF promoter driven GFP expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5XhoI
promoterTEF1 promoter
sequencing_primer_3yGFP-R
sequencing_primer_5M13pUC-fwd
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYPR080W
geneTEF1
id856195
genbank_ids
mutation
nameTEF1 promoter
shRNA_sequence
size
species
4932
Saccharomyces cerevisiae
tags
locationC terminal on backbone
tagyEGFPCLN2PEST (destabilized yeast EGFP)
plasmid copy :
Euroscarf
resistance markers :
1837
tags :
High Copy
terms :
URA3
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA