This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pAc- His- zfTpo
catalog :
64309
citations: 3
Reference
Hason M, Mikulášová T, Machonova O, Pombinho A, van Ham T, Irion U, et al. M-CSFR/CSF1R signaling regulates myeloid fates in zebrafish via distinct action of its receptors and ligands. Blood Adv. 2022;6:1474-1488 pubmed publisher
Svoboda O, Stachura D, Machonova O, Zon L, Traver D, Bartunek P. Ex vivo tools for the clonal analysis of zebrafish hematopoiesis. Nat Protoc. 2016;11:1007-20 pubmed publisher
Svoboda O, Stachura D, Machonova O, Pajer P, Brynda J, Zon L, et al. Dissection of vertebrate hematopoiesis using zebrafish thrombopoietin. Blood. 2014;124:220-8 pubmed publisher
product information
Catalog Number :
64309
Product Name :
pAc- His- zfTpo
article :
doi10.1182/blood-2014-03-564682
id28229332
pubmed_id24869937
bacterial resistance :
Ampicillin
cloning :
backbonepAcGP67- B
backbone_mutation
backbone_originBD Biosciences
backbone_size9765
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth strain :
Express soluble His-tagged zebrafish thrombopoietin in insect cells
origin :
37
pi :
alt_names
tpo
thpo
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5NdeI
promoter
sequencing_primer_3
sequencing_primer_5AAA TGA TAA CCA TCT CGC
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesT, tpo
genethpo
id100189608
genbank_ids
EU267076.1
mutationThrombopoietin starts at AA37 (AA1-36 encode the signal peptide which is not present in the mature secreted protein)
nameThrombopoietin
shRNA_sequence
size
species
7955
Danio rerio
tags
locationN terminal on insert
tag(His)6
resistance markers :
2399
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA