pBa-FRB-3myc-KIF1B beta tail 387-1770 | Addgene 64287 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBa-FRB-3myc-KIF1B beta tail 387-1770

catalog :

64287

citations: 1

Reference
Bentley M, Decker H, Luisi J, Banker G. A novel assay reveals preferential binding between Rabs, kinesins, and specific endosomal subpopulations. J Cell Biol. 2015;208:273-81 pubmed publisher

product information

Catalog Number :

64287

Product Name :

pBa-FRB-3myc-KIF1B beta tail 387-1770

article :

doi	10.1083/jcb.201408056
id	9792
pubmed_id	25624392

bacterial resistance :

Ampicillin

cloning :

backbone

pBa-FRB

backbone_mutation

backbone_origin

backbone_size

6287

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination. XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.

growth strain :

KIF1B beta aa 387-1770 with N-terminal FRB and triple myc tags

origin :

pi :

alt_names

KIF1B v2

cloning

clone_method	Restriction Enzyme
cloning_site_3	BclI
cloning_site_5	BsiWI
promoter	chicken Beta Actin
sequencing_primer_3	GATGAGTTTGGACAAACCAC
sequencing_primer_5	TTCGGCTTCTGGCGTGTGAC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	A530096N05Rik, AI448212, AI506502, D4Mil, D4Mil1e, KIF1Bp1, KIF1Bp130, KIF1Bp2, KIF1Bp204
gene	Kif1b
id	16561

genbank_ids

NM_207682

mutation

aa 387-1770, small portion of 5'end matches rat, 3'end matches mouse. V407A, L536I, S542N, Y939C, Q1093R, T1187I, K1691E, and S1766N relative to NM_207682

name

KIF1B beta

shRNA_sequence

size

4282

species

10090

Mus musculus

10116

Rattus norvegicus