pBa-FRB-3myc-KIF1A tail 391-1698 | Addgene 64286 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pBa-FRB-3myc-KIF1A tail 391-1698

catalog :

64286

citations: 2

Reference
Schelski M, Bradke F. Microtubule retrograde flow retains neuronal polarization in a fluctuating state. Sci Adv. 2022;8:eabo2336 pubmed publisher
Bentley M, Decker H, Luisi J, Banker G. A novel assay reveals preferential binding between Rabs, kinesins, and specific endosomal subpopulations. J Cell Biol. 2015;208:273-81 pubmed publisher

product information

Catalog Number :

64286

Product Name :

pBa-FRB-3myc-KIF1A tail 391-1698

article :

doi	10.1083/jcb.201408056
id	9792
pubmed_id	25624392

bacterial resistance :

Ampicillin

cloning :

backbone

pBa

backbone_mutation

backbone_origin

backbone_size

5863

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination. XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.

growth strain :

KIF1A aa 391-1698 with N-terminal FRB and triple myc tags

origin :

pi :

alt_names

FRB-3myc-Kif1A tail

cloning

clone_method	Restriction Enzyme
cloning_site_3	HpaI
cloning_site_5	AscI
promoter	chicken Beta Actin
sequencing_primer_3	GATGAGTTTGGACAAACCAC
sequencing_primer_5	TTCGGCTTCTGGCGTGTGAC
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	A, ATSV, C630002N23Rik, Gm1626, Kn, Kns1
gene	Kif1a
id	16560

aliases
gene	Kif1a
id	363288

genbank_ids

NM_008440

XM_003750741

mutation

aa 391-1698, 5'end mostly matches rat, 3'end mostly matches mouse

name

KIF1A

shRNA_sequence

size

4502

species

10090

Mus musculus

10116

Rattus norvegicus

32644

Other