pFETCh_GABPA | Addgene 64253 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pFETCh_GABPA

catalog :

64253

citations: 2

Reference
Charpentier M, Khedher A, Menoret S, Brion A, Lamribet K, Dardillac E, et al. CtIP fusion to Cas9 enhances transgene integration by homology-dependent repair. Nat Commun. 2018;9:1133 pubmed publisher
Savic D, Partridge E, Newberry K, Smith S, Meadows S, Roberts B, et al. CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res. 2015;25:1581-9 pubmed publisher

product information

Catalog Number :

64253

Product Name :

pFETCh_GABPA

article :

doi	10.1101/gr.193540.115
id	15802
pubmed_id	26355004

bacterial resistance :

Kanamycin

cloning :

backbone

pFETCH_Donor

backbone_mutation

backbone_origin

backbone_size

5771

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

This plasmid has been used along with PX548_GABPA_1 and PX548_GABPA_2 to add a FLAG tag to human GABPA in HepG2 and/or MCF7 cells by the Mendenhall and Myers labs at HudsonAlpha Institute for Biotechnology/UAH Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.

growth strain :

Homology Arms and 3X Flag with P2A Neo for 3' tagging of human GABPA

origin :

pi :

alt_names

GABPA

NRF2

NFT2

cloning

clone_method	Gibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3	aactgttgggaagggcgatc
sequencing_primer_5	acgcctgtgaaaccgtacta
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	E4TF1-60, E4TF1A, NFT2, NRF2, NRF2A, RCH04A07
gene	GABPA
id	2551

genbank_ids

GABPA

mutation

name

GABPA Homology Arms

shRNA_sequence

size

1600

species

9606

Homo sapiens

32644

Other