This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Cry2 mCherry PGK-1 in pcDNA3.1
catalog :
64214
citations: 1
Reference
O Neill P, Gautam N. Subcellular optogenetic inhibition of G proteins generates signaling gradients and cell migration. Mol Biol Cell. 2014;25:2305-14 pubmed publisher
product information
Catalog Number :
64214
Product Name :
Cry2 mCherry PGK-1 in pcDNA3.1
article :
doi10.1091/mbc.E14-04-0870
id9956
pubmed_id24920824
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_originInvitrogen
backbone_size5428
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
PGK1 is cloned within the KpnI and XbaI sites
growth strain :
mCherry fused between Cry2 and PGK-1 to study cell migration
origin :
37
pi :
alt_names
Cryptochrome 2
cloning
clone_methodRestriction Enzyme
cloning_site_3SmaI
cloning_site_5NheI
promoterCMV
sequencing_primer_3na
sequencing_primer_5T7
site_3_destroyed1
site_5_destroyed
entrez_gene
aliasesAT1G04400, AT-PHH1, ATCRY2, CRYPTOCHROME 2 APOPROTEIN, F19P19.14, F19P19_14, FHA, PHH1, cryptochrome 2
geneCRY2
id839529
genbank_ids
mutation
nameCry2 PHR
shRNA_sequence
size1494
species
3702
Arabidopsis thaliana
tags
locationC terminal on insert
tagmcherry
alt_names
PGKA; MIG10
cloning
clone_methodRestriction Enzyme
cloning_site_3XbaI
cloning_site_5KpnI
promoter
sequencing_primer_3BGH
sequencing_primer_5na
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHEL-S-68p, MIG10, PGKA
genePGK1
id5230
genbank_ids
NM_000291.3
mutation
namePGK1
shRNA_sequence
size1254
species
9606
Homo sapiens
tags
resistance markers :
1218
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA