This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pBa-KIF5C 559-tdTomato-FKBP
catalog :
64211
citations: 4
Reference
Semesta K, Garces A, Tsvetanova N. The psychosis risk factor RBM12 encodes a novel repressor of GPCR/cAMP signal transduction. bioRxiv. 2023;: pubmed publisher
Lim L, Pakan J, Selten M, Marques Smith A, Llorca A, Bae S, et al. Optimization of interneuron function by direct coupling of cell migration and axonal targeting. Nat Neurosci. 2018;21:920-931 pubmed publisher
Thillaiappan N, Chavda A, Tovey S, Prole D, Taylor C. Ca2+ signals initiate at immobile IP3 receptors adjacent to ER-plasma membrane junctions. Nat Commun. 2017;8:1505 pubmed publisher
Bentley M, Decker H, Luisi J, Banker G. A novel assay reveals preferential binding between Rabs, kinesins, and specific endosomal subpopulations. J Cell Biol. 2015;208:273-81 pubmed publisher
product information
Catalog Number :
64211
Product Name :
pBa-KIF5C 559-tdTomato-FKBP
article :
doi10.1083/jcb.201408056
id9792
pubmed_id25624392
bacterial resistance :
Ampicillin
cloning :
backbonepBa.KIF5C 1-559.eGFP
backbone_mutationGFP removed and tdTomato-FKBP added
backbone_originBanker lab (Addgene plasmid #45059)
backbone_size8410
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
pBa vector is susceptable to recombination and should not be grown in fast growing bacteria or cloned using recombination. XL 10-Gold, Stbl3, OmniMax2, DH5alpha, and XL 1-Blue are suitable growth strains.
growth strain :
KIF5C motor protein aa1-559 with C-terminal tandem dimer Tomato and FKBP tags
origin :
37
pi :
alt_names
Kif5c560
kinesin family member 5C
cloning
clone_methodRestriction Enzyme
cloning_site_3HpaI
cloning_site_5AscI
promoterchicken Beta Actin
sequencing_primer_3GATGAGTTTGGACAAACCAC
sequencing_primer_5TTCGGCTTCTGGCGTGTGAC
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneKif5c
id311024
genbank_ids
NM_001107730.1
mutationmotor domain aa1-559
nameKif5c
shRNA_sequence
size1676
species
10116
Rattus norvegicus
tags
locationC terminal on insert
tagtdTomato
locationC terminal on insert
tagFKBP
plasmid copy :
Kif 5c received from Bruce Schnapp, Oregon Health & Science University
resistance markers :
1566
3547
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA