This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mcherry Gamma 9 ---pcDNA3.1
catalog :
64203
citations: 1
Reference
Karunarathne W, Giri L, Kalyanaraman V, Gautam N. Optically triggering spatiotemporally confined GPCR activity in a cell and programming neurite initiation and extension. Proc Natl Acad Sci U S A. 2013;110:E1565-74 pubmed publisher
product information
Catalog Number :
64203
Product Name :
mcherry Gamma 9 ---pcDNA3.1
article :
doi10.1073/pnas.1220697110
id9723
pubmed_id23479634
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1
backbone_mutation
backbone_originInvitrogen
backbone_size5428
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
mCherry was cloned into the HindIII- KpnI sites of pcDNA3.1 and Gamma cone was cloned into the KpnI--EcoR1 sites of pcDNA3.1. Gamma has a His-tag on its N terminus.
growth strain :
G protein Gamma cone(9) subunit tagged with mcherry on the N terminus
origin :
37
pi :
alt_names
Gng9
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoR1
cloning_site_5HindIII
promoterCMV
sequencing_primer_3BGH reverse
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesG-GAMMA-8, G-GAMMA-C, GNG9, GNGT8, HG3I
geneGNGT2
id2793
genbank_ids
NM_174328.2
mutation
nameG protein Gamma 9
shRNA_sequence
size219
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmcherry
locationN terminal on backbone
tag6xHis
resistance markers :
1218
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA