This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFETCh_RAD21
catalog :
64056
citations: 1
Reference
Savic D, Partridge E, Newberry K, Smith S, Meadows S, Roberts B, et al. CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Genome Res. 2015;25:1581-9 pubmed publisher
product information
Catalog Number :
64056
Product Name :
pFETCh_RAD21
article :
doi10.1101/gr.193540.115
id15802
pubmed_id26355004
bacterial resistance :
Kanamycin
cloning :
backbonepFETCH_Donor
backbone_mutation
backbone_origin
backbone_size5771
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
growth notes :
This plasmid has been used along with PX548_RAD21_1 to add a FLAG tag to human RAD21 in HepG2 and MCF7 cells by the Mendenhall and Myers labs at HudsonAlpha Institute for Biotechnology/UAH. Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
growth strain :
Homology Arms and 3X Flag with P2A Neo for 3' tagging of human RAD21
origin :
37
pi :
alt_names
RAD21
SCC1
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3aactgttgggaagggcgatc
sequencing_primer_5acgcctgtgaaaccgtacta
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCDLS4, HR21, HRAD21, MCD1, MGS, NXP1, SCC1, hHR21
geneRAD21
id5885
genbank_ids
mutation
nameRAD21 Homology Arms
shRNA_sequence
size1600
species
9606
Homo sapiens
32644
Other
tags
locationC terminal on insert
tag3X Flag P2A Neo
resistance markers :
1728
2438
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA