This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLenti-FLAG-Akt2-CA
catalog :
64050
citations: 1
Reference
Lim C, Bi X, Wu D, Kim J, Gunning P, Hong W, et al. Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling. Nat Commun. 2015;6:5951 pubmed publisher
product information
Catalog Number :
64050
Product Name :
pLenti-FLAG-Akt2-CA
article :
doi10.1038/ncomms6951
id9911
pubmed_id25575350
bacterial resistance :
Ampicillin
cloning :
backbonepLenti-hiko
backbone_mutation
backbone_origin
backbone_size9124
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
Lentiviral
growth notes :
The MYR sequence was inserted in frame at EcoRI/NheI site into N terminal of pLenti-FLAG-hAkt2 Please cite the following article: Lim, C.-Y. et al. Tropomodulin3 is a novel Akt2 effector regulating insulin-stimulated GLUT4 exocytosis through cortical actin remodeling. Nat Commun 6, 5951 (2015).
growth strain :
Myristoylated-FLAG-tagged Akt2, constitutively active
origin :
37
pi :
alt_names
PKB beta
v-akt murine thymoma viral oncogene homolog 2
RAC-BETA
cloning
clone_methodRestriction Enzyme
cloning_site_3HpaI
cloning_site_5NheI
promoterUbiquitin
sequencing_primer_3
sequencing_primer_5GGCGAGTGTGTTTTGTGAAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesHIHGHH, PKBB, PKBBETA, PRKBB, RAC-BETA
geneAKT2
id208
genbank_ids
NM_001626.5
mutation
nameAKT2
shRNA_sequence
size1446
species
9606
Homo sapiens
tags
locationN terminal on insert
tagFLAG
locationN terminal on backbone
tagMYR
plasmid copy :
human AKT2 sequence was derived from Addgene #9016
resistance markers :
2289
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA