This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3-EngHD
catalog :
64033
citations: 1
Reference
Christophe Hobertus C, Christophe D. Human Thyroid Oxidases genes promoter activity in thyrocytes does not appear to be functionally dependent on Thyroid Transcription Factor-1 or Pax8. Mol Cell Endocrinol. 2007;264:157-63 pubmed
product information
Catalog Number :
64033
Product Name :
pcDNA3-EngHD
article :
doi10.1016/j.mce.2006.11.005
id9905
pubmed_id17182173
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3
backbone_mutation
backbone_originInvitrogen
backbone_size5446
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
There is a BamHI site in between the Eng and HD coding sequences. Drosophila Engrailed (Eng)contains H22P and L158P compared to closest reference sequence (AAA65478.1). Depositor states that these mutation should not affect function.
growth strain :
Mammalian expression of TTF-1 homeodomain fused to the N-terminal repressor domain of the drosophila engrailed protein (Eng)
origin :
37
pi :
alt_names
T/EBP
Nkx-2.1
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3BGH Reverse
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneTTF1
id608462
genbank_ids
X77910
mutationhomeodomain, aa 154-226
nameTTF-1
shRNA_sequence
size
species
32644
Other
dog
tags
locationN terminal on insert
tagEng (N-terminal repressor domain of the drosophila engrailed protein)
resistance markers :
2284
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA