This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pSG 25
catalog :
63867
citations: 2
Reference
Gupta A, Lentzsch A, Siegel A, Yu Z, Chio U, Cheng Y, et al. Dodecamer assembly of a metazoan AAA+ chaperone couples substrate extraction to refolding. Sci Adv. 2023;9:eadf5336 pubmed publisher
O Connor M, Dahlberg A. Mutations at U2555, a tRNA-protected base in 23S rRNA, affect translational fidelity. Proc Natl Acad Sci U S A. 1993;90:9214-8 pubmed
product information
Catalog Number :
63867
Product Name :
pSG 25
article :
doi
id9874
pubmed_id8415679
bacterial resistance :
Tetracycline
cloning :
backbonepACYC184
backbone_mutation
backbone_origin
backbone_size4500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
pSG25 expresses very high levels of beta-galactosidase in E. coli. Various mutant derivatives are used to measure translational errors.
growth strain :
Expression of beta-galactosidase at high levels in E. coli
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3ScaI
cloning_site_5NaeI
promoterPtacI
sequencing_primer_3
sequencing_primer_5M13pUC-Rev
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesb0344, ECK0341
genelacZ
id945006
genbank_ids
NC_000913.3
mutation
namelacZ
shRNA_sequence
size3200
species
32644
Other
Escherichia coli
tags
plasmid copy :
The lacZ gene originated from pKK-CUG (Bob Weiss; Wagner et al. 1994 J. Bact. 176:1683)
resistance markers :
2279
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA