Mishler2014-No-aptamer-controlluciferase-pUC19 | Addgene 63856 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

Mishler2014-No-aptamer-controlluciferase-pUC19

catalog :

63856

citations: 1

Reference
Espah Borujeni A, Mishler D, Wang J, Huso W, Salis H. Automated physics-based design of synthetic riboswitches from diverse RNA aptamers. Nucleic Acids Res. 2016;44:1-13 pubmed publisher

product information

Catalog Number :

63856

Product Name :

Mishler2014-No-aptamer-controlluciferase-pUC19

article :

doi	10.1093/nar/gkv1289
id	16363
pubmed_id	26621913

bacterial resistance :

Ampicillin

cloning :

backbone

pUC19

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Synthetic Biology

growth strain :

This pUC19 vector contains a no-aptamer control mRNA encoding luciferase gene. It should be used when testing AEB-TMR7 and AEB-TMR10 riboswitches (all on pUC19) to measure their non-specific ligand effect on luciferase expression.

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	unknown
cloning_site_5	unknown
promoter	tac
sequencing_primer_3
sequencing_primer_5	LucNRev
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

No-aptamer control mRNA

shRNA_sequence

size

species

100

Synthetic