This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pMT-IMD-V5-His
catalog :
63748
product information
Catalog Number :
63748
Product Name :
pMT-IMD-V5-His
article :
doi
id9859
pubmed_id
bacterial resistance :
Ampicillin
cloning :
backbonepMT-V5-His
backbone_mutationnone
backbone_originlifetechnologies
backbone_size3500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
*Note: Addgene's quality control sequencing finds two amino acid residue substitutions, L47M and H212Y, in the Drosophila IMD insert. These residue changes are not thought to affect plasmid function, and the protein has been shown to activate the Imd pathway as expected.
growth strain :
Expresses drosophila IMD tagged with V5 and 6xHis epitopes, under control of metallothionein promoter
origin :
37
pi :
alt_names
IMD
cloning
clone_methodRestriction Enzyme
cloning_site_3XhOI
cloning_site_5EcoRI
promoterpMT
sequencing_primer_3TAGAAGGCACAGTCGAGG
sequencing_primer_5 CATCTCAGTGCAACTAAA
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesDmel_CG5576, BG5, CG5576, Dmel CG5576, IMD, IMd, Imd, anon-WO0172774.166, shadok
geneimd
id44339
genbank_ids
mutation*see comment below
nameimmune deficiency
shRNA_sequence
size
species
7227
Drosophila melanogaster
tags
locationC terminal on backbone
tagV5-6XHis
resistance markers :
2274
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA