This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MIP 339 CoMiP coLin28 2A Nanog IRES eGFP
catalog :
63729
citations: 1
Reference
Diecke S, Lu J, Lee J, Termglinchan V, Kooreman N, Burridge P, et al. Novel codon-optimized mini-intronic plasmid for efficient, inexpensive, and xeno-free induction of pluripotency. Sci Rep. 2015;5:8081 pubmed publisher
product information
Catalog Number :
63729
Product Name :
MIP 339 CoMiP coLin28 2A Nanog IRES eGFP
article :
doi10.1038/srep08081
id9847
pubmed_id25628230
bacterial resistance :
Other
cloning :
backboneno backbone--uses MiP intron for replication in bacteria
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
In addition to the publication listed above, more details can be found in Lu, et al. Mol Ther. 2013, PMID 23459514.
growth strain :
To express codon optimized Lin-28 and wild type Nanog. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).
inserts :
RNA-OUT selection competent cells, such as NTC4862 DH5 competent cells (Nature Technology)
origin :
30
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterSFFV
sequencing_primer_3
sequencing_primer_5unknown
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCSDD1, LIN-28, LIN28, ZCCHC1, lin-28A
geneLIN28A
id79727
genbank_ids
mutationcodon optimized
nameLin28
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationC terminal on insert
tagT2A
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoter
sequencing_primer_3
sequencing_primer_5unknown
site_3_destroyed
site_5_destroyed
entrez_gene
aliases
geneNANOG
id79923
genbank_ids
mutationcodon optimized
nameNanog
shRNA_sequence
size
species
9606
Homo sapiens
tags
plasmid copy :
The pUC-RNA-OUT sequence used as MIP intron was cloned from NTC vector 8682.
resistance markers :
2272
1011
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA