This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
MIP 227 CoMiP 4in1 wo shRNA p53
catalog :
63727
citations: 1
Reference
Diecke S, Lu J, Lee J, Termglinchan V, Kooreman N, Burridge P, et al. Novel codon-optimized mini-intronic plasmid for efficient, inexpensive, and xeno-free induction of pluripotency. Sci Rep. 2015;5:8081 pubmed publisher
product information
Catalog Number :
63727
Product Name :
MIP 227 CoMiP 4in1 wo shRNA p53
article :
doi10.1038/srep08081
id9847
pubmed_id25628230
bacterial resistance :
Other
cloning :
backbonecodon-optimized minicircle (CoMiC) construct
backbone_mutationcodon-optimized expression cassette of the four canonical reprogramming factors OCT4, KLF4, SOX2, and c-MYC (OKSM)
backbone_origin
backbone_size7608
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
In addition to the publication listed above, more details can be found in Lu, et al. Mol Ther. 2013, PMID 23459514.
growth strain :
To express Oct4, Klf4, Sox2, and c-Myc. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).
inserts :
RNA-OUT selection competent cells, such as NTC4862 DH5 competent cells (Nature Technology)
origin :
30
pi :
alt_names
pUC-RNA-OUT
MIP intron
cloning
clone_methodRestriction Enzyme
cloning_site_3AgeI
cloning_site_5AgeI
promoterSFFV
sequencing_primer_3
sequencing_primer_5unknown
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesOCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
genePOU5F1
id5460
aliasesEZF, GKLF
geneKLF4
id9314
aliasesANOP3, MCOPS3
geneSOX2
id6657
aliasesMRTL, MYCC, bHLHe39, c-Myc
geneMYC
id4609
genbank_ids
mutationcodon-optimised
nameMini-intronic-plasmid intron
shRNA_sequence
size1355
species
9606
Homo sapiens
tags
locationC terminal on backbone
tagIRES-dtomato
plasmid copy :
The pUC-RNA-OUT sequence used as MIP intron was cloned from NTC vector 8682.
resistance markers :
2272
1011
tags :
High Copy
terms :
Other
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA