MIP 247 CoMiP 4in1 with shRNA p53 | Addgene 63726 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

MIP 247 CoMiP 4in1 with shRNA p53

catalog :

63726

citations: 10

Reference
Jin W, He Y, Li T, Long F, Qin X, Yuan Y, et al. Rapid and robust derivation of mesenchymal stem cells from human pluripotent stem cells via temporal induction of neuralized ectoderm. Cell Biosci. 2022;12:31 pubmed publisher
Zywitza V, Rusha E, Shaposhnikov D, Ruiz Orera J, Telugu N, Rishko V, et al. Naïve-like pluripotency to pave the way for saving the northern white rhinoceros from extinction. Sci Rep. 2022;12:3100 pubmed publisher
Lutter G, Puehler T, Cyganek L, Seiler J, Rogler A, Herberth T, et al. Biodegradable Poly-ε-Caprolactone Scaffolds with ECFCs and iMSCs for Tissue-Engineered Heart Valves. Int J Mol Sci. 2022;23: pubmed publisher
Huang M, Yang Z, Li Y, Lan H, Cyganek L, Yuecel G, et al. Dopamine D1/D5 Receptor Signaling Is Involved in Arrhythmogenesis in the Setting of Takotsubo Cardiomyopathy. Front Cardiovasc Med. 2021;8:777463 pubmed publisher
Kozisek T, Hamann A, Samuelson L, Fudolig M, Pannier A. Comparison of promoter, DNA vector, and cationic carrier for efficient transfection of hMSCs from multiple donors and tissue sources. Mol Ther Nucleic Acids. 2021;26:81-93 pubmed publisher
Khadjeh S, Hindmarsh V, Weber F, Cyganek L, Vidal R, Torkieh S, et al. CRISPLD1: a novel conserved target in the transition to human heart failure. Basic Res Cardiol. 2020;115:27 pubmed publisher
El Battrawy I, Müller J, Zhao Z, Cyganek L, Zhong R, Zhang F, et al. Studying Brugada Syndrome With an SCN1B Variants in Human-Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Front Cell Dev Biol. 2019;7:261 pubmed publisher
Zhao Z, Lan H, El Battrawy I, Li X, Buljubasic F, Sattler K, et al. Ion Channel Expression and Characterization in Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes. Stem Cells Int. 2018;2018:6067096 pubmed publisher
El Battrawy I, Zhao Z, Lan H, Schünemann J, Sattler K, Buljubasic F, et al. Estradiol protection against toxic effects of catecholamine on electrical properties in human-induced pluripotent stem cell derived cardiomyocytes. Int J Cardiol. 2018;254:195-202 pubmed publisher
Diecke S, Lu J, Lee J, Termglinchan V, Kooreman N, Burridge P, et al. Novel codon-optimized mini-intronic plasmid for efficient, inexpensive, and xeno-free induction of pluripotency. Sci Rep. 2015;5:8081 pubmed publisher

product information

Catalog Number :

63726

Product Name :

MIP 247 CoMiP 4in1 with shRNA p53

article :

doi	10.1038/srep08081
id	9847
pubmed_id	25628230

bacterial resistance :

Other

cloning :

backbone

codon-optimized minicircle (CoMiC) construct

backbone_mutation

codon-optimized expression cassette of the four canonical reprogramming factors OCT4, KLF4, SOX2, and c-MYC (OKSM) described previously, and combined this cassette with shRNA targeting p53

backbone_origin

Joseph Wu's Lab at Stanford University

backbone_size

8062

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

In addition to the publication listed above, more details can be found in Lu, et al. Mol Ther. 2013, PMID 23459514.

growth strain :

To express Oct4, Klf4, Sox2, c-Myc and hairpin RNA p53. A single plasmid reprogramming system using a mini-intronic plasmid (MIP).

inserts :

RNA-OUT selection competent cells, such as NTC4862 DH5 competent cells (Nature Technology)

origin :

pi :

alt_names

pUC-RNA-OUT

MIP intron

cloning

clone_method	Restriction Enzyme
cloning_site_3	AgeI
cloning_site_5	AgeI
promoter	SFFV for Pct4, Klf4, Sox2 and c-myc; U6 for p53
sequencing_primer_3
sequencing_primer_5	unknown
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	OCT3, OCT4, OTF-3, OTF3, OTF4, Oct-3, Oct-4, Oct3/4
gene	POU5F1
id	5460

aliases	EZF, GKLF
gene	KLF4
id	9314

aliases	ANOP3, MCOPS3
gene	SOX2
id	6657

aliases	MRTL, MYCC, bHLHe39, c-Myc
gene	MYC
id	4609

aliases	BCC7, BMFS5, LFS1, P53, TRP53
gene	TP53
id	7157

genbank_ids

mutation

codon-optimised

name

Mini-intronic-plasmid intron

shRNA_sequence

size

1355

species

9606

Homo sapiens