This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pcDNA3.1-FAM20C:V5
catalog :
63584
citations: 1
Reference
Ishikawa H, Xu A, Ogura E, Manning G, Irvine K. The Raine syndrome protein FAM20C is a Golgi kinase that phosphorylates bio-mineralization proteins. PLoS ONE. 2012;7:e42988 pubmed publisher
product information
Catalog Number :
63584
Product Name :
pcDNA3.1-FAM20C:V5
article :
doi10.1371/journal.pone.0042988
id9798
pubmed_id22900076
bacterial resistance :
Ampicillin
cloning :
backbonepcDNA3.1/V5-HisB
backbone_mutation
backbone_originLife Technologies
backbone_size5500
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Full length mouse FAM20C extending 98 bp upstream of the ATG and excluding the stop codon, was amplified from FAM20C mouse BC025826 cDNA by PCR using primers FAM20C-HindIII-F and FAM20C-NotI-R and the resulting fragment was cloned into HindIII and NotI cut pcDNA3.1/V5-His-B to create above plasmid.
growth strain :
FAM20C can phosphorylate secreted phosphoproteins, and characterization of its activity identifies FAM20C as a Golgi casein kinase.
origin :
37
pi :
alt_names
family with sequence similarity 20, member C
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5HindIII
promoterCMV
sequencing_primer_3BGH-rev
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC76981, DMP-4, DMP4, GEF-CK, mKIAA4081
geneFam20c
id80752
genbank_ids
mutation
nameFAM20C
shRNA_sequence
size1900
species
10090
Mus musculus
tags
locationC terminal on backbone
tagV5
locationC terminal on backbone
tag6xHis
resistance markers :
2571
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA