This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pFastbac1-GST-hUSP7
catalog :
63573
citations: 3
Reference
Chatrin C, Gabrielsen M, Buetow L, Nakasone M, Ahmed S, Sumpton D, et al. Structural insights into ADP-ribosylation of ubiquitin by Deltex family E3 ubiquitin ligases. Sci Adv. 2020;6: pubmed publisher
Czech Sioli M, Siebels S, Radau S, Zahedi R, Schmidt C, Dobner T, et al. The Ubiquitin-Specific Protease Usp7, a Novel Merkel Cell Polyomavirus Large T-Antigen Interaction Partner, Modulates Viral DNA Replication. J Virol. 2020;94: pubmed publisher
Faesen A, Dirac A, Shanmugham A, Ovaa H, Perrakis A, Sixma T. Mechanism of USP7/HAUSP activation by its C-terminal ubiquitin-like domain and allosteric regulation by GMP-synthetase. Mol Cell. 2011;44:147-59 pubmed publisher
product information
Catalog Number :
63573
Product Name :
pFastbac1-GST-hUSP7
article :
doi10.1016/j.molcel.2011.06.034
id9794
pubmed_id21981925
bacterial resistance :
Ampicillin
cloning :
backbonepFastBac1
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Insect Expression
growth notes :
Please note that the USP7 gene in this plasmid contains mutations S600P, F679L and V1020A when compared to the annotated gene/protein sequences within the Genbank/Uniprot databases. This plasmid should function as described in the associated publication.
growth strain :
Expression of synthetic human USP7 tagged with GST
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5BamHI
promoterPolyhedrin
sequencing_primer_3SV40 poly A Reverse
sequencing_primer_5Polyhedrin Forward (AAATGATAACCATCTCGC)
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesC16DELp13.2, DEL16P13.2, HAFOUS, HAUSP, TEF1
geneUSP7
id7874
genbank_ids
mutationSynthetic based on human protein sequence (please see depositor comment section below)
nameUSP7
shRNA_sequence
size3309
species
9606
Homo sapiens
100
Synthetic
tags
locationN terminal on insert
tagGST
resistance markers :
2258
tags :
Unknown
terms :
Gentamicin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA