This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET3a-hUBA1
catalog :
63571
citations: 8
Reference
Wu K, Pan Z. A Novel Strategy to Track Lysine-48 Ubiquitination by Fluorescence Resonance Energy Transfer. Methods Mol Biol. 2021;2267:91-102 pubmed publisher
Kudriaeva A, Saratov G, Kaminskaya A, Vladimirov V, Barzilovich P, Belogurov A. Polyamines Counteract Carbonate-Driven Proteasome Stalling in Alkaline Conditions. Biomolecules. 2020;10: pubmed publisher
Fenteany G, Gaur P, Sharma G, Pintér L, Kiss E, Haracska L. Robust high-throughput assays to assess discrete steps in ubiquitination and related cascades. BMC Mol Cell Biol. 2020;21:21 pubmed publisher
Fenteany G, Gaur P, Hegedus L, Dudás K, Kiss E, Wéber E, et al. Multilevel structure-activity profiling reveals multiple green tea compound families that each modulate ubiquitin-activating enzyme and ubiquitination by a distinct mechanism. Sci Rep. 2019;9:12801 pubmed publisher
de Oliveira J, do Prado P, da Costa S, Sforça M, Canateli C, Ranzani A, et al. Mechanistic insights revealed by a UBE2A mutation linked to intellectual disability. Nat Chem Biol. 2019;15:62-70 pubmed publisher
Vanarotti M, Evison B, Actis M, Inoue A, McDonald E, Shao Y, et al. Small-molecules that bind to the ubiquitin-binding motif of REV1 inhibit REV1 interaction with K164-monoubiquitinated PCNA and suppress DNA damage tolerance. Bioorg Med Chem. 2018;26:2345-2353 pubmed publisher
Wu K, Chong R, Yu Q, Bai J, Spratt D, Ching K, et al. Suramin inhibits cullin-RING E3 ubiquitin ligases. Proc Natl Acad Sci U S A. 2016;113:E2011-8 pubmed publisher
Pichler A, Knipscheer P, Oberhofer E, van Dijk W, Körner R, Olsen J, et al. SUMO modification of the ubiquitin-conjugating enzyme E2-25K. Nat Struct Mol Biol. 2005;12:264-9 pubmed
product information
Catalog Number :
63571
Product Name :
pET3a-hUBA1
article :
doi10.1038/nsmb903
id9793
pubmed_id15723079
bacterial resistance :
Ampicillin
cloning :
backbonepET
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Expression of human UBA1 tagged with His
origin :
37
pi :
alt_names
Ubiquitin-activating enzyme E1
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NdeI
promoterT7
sequencing_primer_3T7 Terminal
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesA1S9, A1S9T, A1ST, AMCX1, CFAP124, GXP1, POC20, SMAX2, UBA1A, UBE1, UBE1X, VEXAS
geneUBA1
id7317
genbank_ids
mutationIsoform 2 (1018 amino acids)
nameUBA1
shRNA_sequence
size3087
species
9606
Homo sapiens
32644
Other
tags
resistance markers :
2258
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA