pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R | Addgene 63565 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R

catalog :

63565

citations: 3

Reference
Palminha N, dos Santos Souza C, Griffin J, Liao C, Ferraiuolo L, El Khamisy S. Defective repair of topoisomerase I induced chromosomal damage in Huntington's disease. Cell Mol Life Sci. 2022;79:160 pubmed publisher
Velimezi G, Robinson Garcia L, Muñoz Martínez F, Wiegant W, Ferreira da Silva J, Owusu M, et al. Map of synthetic rescue interactions for the Fanconi anemia DNA repair pathway identifies USP48. Nat Commun. 2018;9:2280 pubmed publisher
Mattiroli F, Vissers J, van Dijk W, Ikpa P, Citterio E, Vermeulen W, et al. RNF168 ubiquitinates K13-15 on H2A/H2AX to drive DNA damage signaling. Cell. 2012;150:1182-95 pubmed publisher

product information

Catalog Number :

63565

Product Name :

pCDNA3.1-Flag-H2A K5-9-118-119-125-127-129R

article :

doi	10.1016/j.cell.2012.08.005
id	9791
pubmed_id	22980979

bacterial resistance :

Ampicillin

cloning :

backbone

pcDNA3.1

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth strain :

Expression of human H2A (with multiple Lysine to Arginine point mutations) tagged with Flag

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRV
cloning_site_5	BamHI
promoter	CMV
sequencing_primer_3	BGH-rev
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	H2A, H2A-GL101, H2A/q, H2AFQ, HIST2H2AC
gene	H2AC20
id	8338

genbank_ids

mutation

Lysines at 5, 9, 118, 119, 125, 127, and 129 are now Arginine

name

H2A

shRNA_sequence

size

394

species

9606

Homo sapiens