AAV-FLEX-PKIalpha-IRES-nls-mRuby2 | Addgene 63059 product information

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product summary

company name :

Addgene

product type :

cDNA

product name :

AAV-FLEX-PKIalpha-IRES-nls-mRuby2

catalog :

63059

citations: 3

Reference
Lee S, Lodder B, Chen Y, Patriarchi T, Tian L, Sabatini B. Cell-type-specific asynchronous modulation of PKA by dopamine in learning. Nature. 2021;590:451-456 pubmed publisher
Chen Y, Granger A, Tran T, Saulnier J, Kirkwood A, Sabatini B. Endogenous Gαq-Coupled Neuromodulator Receptors Activate Protein Kinase A. Neuron. 2017;96:1070-1083.e5 pubmed publisher
Chen Y, Saulnier J, Yellen G, Sabatini B. A PKA activity sensor for quantitative analysis of endogenous GPCR signaling via 2-photon FRET-FLIM imaging. Front Pharmacol. 2014;5:56 pubmed publisher

product information

Catalog Number :

63059

Product Name :

AAV-FLEX-PKIalpha-IRES-nls-mRuby2

article :

doi	10.3389/fphar.2014.00056
id	9105
pubmed_id	24765076

bacterial resistance :

Ampicillin

cloning :

backbone

AAV-FLEX-tmeGFP-IRES-nls-mRuby2

backbone_mutation

N/A

backbone_origin

Yao Chen and Bernardo Sabatini

backbone_size

6400

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

AAV

Cre/Lox

growth strain :

Expresses PKIalpha and nuclear localized mRuby2 (linked with IRES) in a Cre-dependent manner in an AAV backbone

growth temp :

Use recombinase-free E. coli (e.g. Invitrogen's Stbl3)

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	BglII
cloning_site_5	AvrII
promoter	CAG
sequencing_primer_3	GAGGTTGATTATCGATAAGC
sequencing_primer_5	GCAACGTGCTGGTTATTGTG
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AI415001, PKIalpha
gene	Pkia
id	18767

genbank_ids

NM_008862

mutation

name

PKIalpha

shRNA_sequence

size

231

species

10090

Mus musculus

100

Synthetic