This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
lgp120+linker-pEGFP N1
catalog :
62963
citations: 1
product information
Catalog Number :
62963
Product Name :
lgp120+linker-pEGFP N1
article :
| doi | |
| id | 9731 |
| pubmed_id |
bacterial resistance :
Kanamycin
cloning :
| backbone | pEGFP-N1 | |
| backbone_mutation | ||
| backbone_origin | ||
| backbone_size | 4700 | |
| promoter | ||
| sequencing_primer_3 | ||
| sequencing_primer_5 | ||
| vector_types |
|
growth notes :
The 10 amino acid linker was designed using the sequence as described below but with different cloning sites (EcoRI and BamHI): Patterson GH and Lippincott-Schwartz J. (2002) A Photoactivatable GFP for Selective Photolabeling of Proteins and Cells. Science 297, 1873-1877. Examples of use: Jahreiss L, Menzies FM, Rubinsztein DC. (2008) The itinerary of autophagosomes: from peripheral formation to kiss-and-run fusion with lysosomes. Traffic 9: 574-587. Renna M, Schaffner C, Winslow AR, Menzies FM, Peden AA, Floto RA, Rubinsztein DC. (2011) Autophagic substrate clearance requires activity of the syntaxin-5 SNARE complex. J Cell Sci 124:469-482. Ogunbayo OA, Zhu Y, Shen B, Agban E, Li J, Ma J, Zhu MX, Evans AM. (2015) Organelle-specific Subunit Interactions of the Vertebrate Two-pore Channel Family. J. Biol. Chem. 290: 1086-1095.
growth strain :
rat lgp120 + 10 amino acid linker cloned into pEGFP N1. The linker separates the targetting motif in lgp120 from EGFP.
origin :
37
pi :
|
resistance markers :
| 2251 |
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
Watertown, MA 02472
info@addgene.org
https://www.addgene.org617.225.9000
headquarters: USA
questions and comments