This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCMV CytoBAS
catalog :
62860
citations: 1
Reference
van der Velden L, Golynskiy M, Bijsmans I, van Mil S, Klomp L, Merkx M, et al. Monitoring bile acid transport in single living cells using a genetically encoded Förster resonance energy transfer sensor. Hepatology. 2013;57:740-52 pubmed publisher
product information
Catalog Number :
62860
Product Name :
pCMV CytoBAS
article :
doi10.1002/hep.26012
id9714
pubmed_id22899095
bacterial resistance :
Kanamycin
cloning :
backbonepECFP-C1
backbone_mutationSynthetic DNA construct encoding BAS-1 was cloned into pECFP-C1, replacing ECFP, to generate NucleoBAS. CytoBAS was created by KpnI digestion of NucleoBAS, which removed the NLS.
backbone_originClontech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Fluorescent bile acid sensor (BAS) that allows spatiotemporal monitoring of bile acid transport in single living cells using FRET
origin :
37
pi :
alt_names
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3SV40pA-R
sequencing_primer_5CMV-F
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameCytoBAS
shRNA_sequence
size
species
100
Synthetic
tags
resistance markers :
2245
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA