pCS2-nCas9n-nanos 3 UTR | Addgene 62542 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pCS2-nCas9n-nanos 3 UTR

catalog :

62542

citations: 4

Reference
Yue Z, Fan Z, Zhang H, Feng B, Wu C, Chen S, et al. Differential roles of the fish chitinous membrane in gut barrier immunity and digestive compartments. EMBO Rep. 2023;24:e56645 pubmed publisher
Lee M, Wan J, Goldman D. Tgfb3 collaborates with PP2A and notch signaling pathways to inhibit retina regeneration. elife. 2020;9: pubmed publisher
Vejnar C, Moreno Mateos M, Cifuentes D, Bazzini A, Giraldez A. Optimized CRISPR-Cas9 System for Genome Editing in Zebrafish. Cold Spring Harb Protoc. 2016;2016: pubmed publisher
Moreno Mateos M, Vejnar C, Beaudoin J, Fernandez J, Mis E, Khokha M, et al. CRISPRscan: designing highly efficient sgRNAs for CRISPR-Cas9 targeting in vivo. Nat Methods. 2015;12:982-8 pubmed publisher

product information

Catalog Number :

62542

Product Name :

pCS2-nCas9n-nanos 3 UTR

article :

doi	10.1038/nmeth.3543
id	15729
pubmed_id	26322839

bacterial resistance :

Ampicillin

cloning :

backbone

pCS2-nCas9n

backbone_mutation

backbone_origin

backbone_size

8272

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

CRISPR

growth notes :

vector backbone was Addgene Plasmid #47929 from Wenbiao Chen

growth strain :

expression of a germ-line targeted, codon optimized Cas9 for genome-editing in zebrafish

origin :

pi :

alt_names

nos1

cloning

clone_method	Restriction Enzyme
cloning_site_3	NotI
cloning_site_5	SnaBI
promoter	SP6
sequencing_primer_3	T3
sequencing_primer_5	ATCaccggtAGCGGACATTGATGCTCC
site_3_destroyed
site_5_destroyed	1

entrez_gene

aliases	cb725, nanos, nanos1, nos1
gene	nanos3
id	140631

genbank_ids

mutation

3' UTR only

name

nanos 3' UTR

shRNA_sequence

size

species

7955

Danio rerio