This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
Actin-mRFP-PAGFP
catalog :
62382
citations: 2
Reference
Bera K, Kiepas A, Godet I, Li Y, Mehta P, Ifemembi B, et al. Extracellular fluid viscosity enhances cell migration and cancer dissemination. Nature. 2022;611:365-373 pubmed publisher
Kueh H, Charras G, Mitchison T, Brieher W. Actin disassembly by cofilin, coronin, and Aip1 occurs in bursts and is inhibited by barbed-end cappers. J Cell Biol. 2008;182:341-53 pubmed publisher
product information
Catalog Number :
62382
Product Name :
Actin-mRFP-PAGFP
article :
doi10.1083/jcb.200801027
id9607
pubmed_id18663144
bacterial resistance :
Kanamycin
cloning :
backboneEGFP-C1
backbone_mutationReplaced EGFP by PAGFP Added mRFP to the N terminal of PAGFP between NheI and AgeI
backbone_originClontech
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses beta actin with both a PAGFP and mRFP tag.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3BamHI
cloning_site_5NheI
promoterCMV
sequencing_primer_3EGFP C rev
sequencing_primer_5CMV forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBKRNS, BNS, BRWS1, CSMH, DDS1, PS1TP5BP1, THC8
geneACTB
id60
genbank_ids
NM_001101.4
mutation
namebeta actin
shRNA_sequence
size1128
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmRFP
locationN terminal on insert
tagPAGFP
resistance markers :
2227
1002
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA