pET28-p53 (1-73) (72R) | Addgene 62082 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pET28-p53 (1-73) (72R)

catalog :

62082

citations: 2

Reference
Schrag L, Liu X, Thevarajan I, Prakash O, Zolkiewski M, Chen J. Cancer-Associated Mutations Perturb the Disordered Ensemble and Interactions of the Intrinsically Disordered p53 Transactivation Domain. J Mol Biol. 2021;433:167048 pubmed publisher
Vise P, Baral B, Latos A, Daughdrill G. NMR chemical shift and relaxation measurements provide evidence for the coupled folding and binding of the p53 transactivation domain. Nucleic Acids Res. 2005;33:2061-77 pubmed

product information

Catalog Number :

62082

Product Name :

pET28-p53 (1-73) (72R)

article :

doi	10.1093/nar/gki336
id	9542
pubmed_id	15824059

bacterial resistance :

Kanamycin

cloning :

backbone

pET28A

backbone_mutation

backbone_origin

Novagen

backbone_size

5639

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

Includes a thrombin cleavage site

growth strain :

Expression of human p53 (1-73) (72R) in e. coli

origin :

pi :

alt_names

p53 (1-73) (72R)

cloning

clone_method	Restriction Enzyme
cloning_site_3	XhoI
cloning_site_5	NdeI
promoter	T7
sequencing_primer_3	T7 term
sequencing_primer_5	T7
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	BCC7, BMFS5, LFS1, P53, TRP53
gene	TP53
id	7157

genbank_ids

mutation

contains TAD residues 1-73; 72R

name

p53

shRNA_sequence

size

219

species

9606

Homo sapiens