This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET28-p53 (1-73) (K24N)
catalog :
62079
citations: 1
Reference
Zhan Y, Wu H, Powell A, Daughdrill G, Ytreberg F. Impact of the K24N mutation on the transactivation domain of p53 and its binding to murine double-minute clone 2. Proteins. 2013;81:1738-47 pubmed publisher
product information
Catalog Number :
62079
Product Name :
pET28-p53 (1-73) (K24N)
article :
doi10.1002/prot.24310
id9541
pubmed_id23609977
bacterial resistance :
Kanamycin
cloning :
backbonepET28A
backbone_mutation
backbone_originNovagen
backbone_size5639
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Includes a thrombin cleavage site
growth strain :
Expression of human p53 (1-73) (K24N) in e. coli
origin :
37
pi :
alt_names
p53 (1-73) (K24N)
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoterT7
sequencing_primer_3T7 term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutationcontains TAD residues 1-73; K24N
namep53
shRNA_sequence
size219
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis
resistance markers :
2211
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA