This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pET28A-p53 (1-73) (P12A P13A)
catalog :
62066
citations: 1
Reference
Borcherds W, Theillet F, Katzer A, Finzel A, Mishall K, Powell A, et al. Disorder and residual helicity alter p53-Mdm2 binding affinity and signaling in cells. Nat Chem Biol. 2014;10:1000-2 pubmed publisher
product information
Catalog Number :
62066
Product Name :
pET28A-p53 (1-73) (P12A P13A)
article :
doi10.1038/nchembio.1668
id9540
pubmed_id25362358
bacterial resistance :
Kanamycin
cloning :
backbonepET28A
backbone_mutation
backbone_originNovagen
backbone_size5639
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Includes a thrombin cleavage site
growth strain :
Expression of human p53 (residues 1-73) (P12A, P13A) in e. coli
origin :
37
pi :
alt_names
p53 residues 1-73, P12A, P13A
p53
cloning
clone_methodRestriction Enzyme
cloning_site_3XhoI
cloning_site_5NdeI
promoterT7
sequencing_primer_3T7 term
sequencing_primer_5T7
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesBCC7, BMFS5, LFS1, P53, TRP53
geneTP53
id7157
genbank_ids
mutationContains TAD residues 1-73; P12A, P13A
namep53
shRNA_sequence
size219
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis
resistance markers :
2211
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA