This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pGEX6P2-MDM2 (17-125)
catalog :
62063
citations: 4
Reference
Schrag L, Liu X, Thevarajan I, Prakash O, Zolkiewski M, Chen J. Cancer-Associated Mutations Perturb the Disordered Ensemble and Interactions of the Intrinsically Disordered p53 Transactivation Domain. J Mol Biol. 2021;433:167048 pubmed publisher
Dowarha D, Chou R, Yu C. S100A1 blocks the interaction between p53 and mdm2 and decreases cell proliferation activity. PLoS ONE. 2020;15:e0234152 pubmed publisher
Morimoto J, Hosono Y, Sando S. Isolation of a peptide containing d-amino acid residues that inhibits the α-helix-mediated p53-MDM2 interaction from a one-bead one-compound library. Bioorg Med Chem Lett. 2018;28:231-234 pubmed publisher
Borcherds W, Theillet F, Katzer A, Finzel A, Mishall K, Powell A, et al. Disorder and residual helicity alter p53-Mdm2 binding affinity and signaling in cells. Nat Chem Biol. 2014;10:1000-2 pubmed publisher
product information
Catalog Number :
62063
Product Name :
pGEX6P2-MDM2 (17-125)
article :
doi10.1038/nchembio.1668
id9540
pubmed_id25362358
bacterial resistance :
Ampicillin
cloning :
backbonePGEX-6P-2
backbone_mutation
backbone_originGE Healthcare Life Sciences
backbone_size4985
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Prescission cleavage.
growth strain :
Expression of human MDM2 (17-125) in e. coli
origin :
37
pi :
alt_names
MDM2 (residues 17-125)
MDM2 proto-oncogene, E3 ubiquitin protein ligase
hdm2
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promotertac
sequencing_primer_3pGEX3
sequencing_primer_5pGEX5
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesACTFS, HDMX, LSKB, hdm2
geneMDM2
id4193
genbank_ids
mutationcontains residues 17-125
nameMDM2
shRNA_sequence
size318
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagGST
plasmid copy :
Includes prescission cleavage site
resistance markers :
2211
tags :
Low Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA