This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)
catalog :
61938
citations: 1
Reference
Vander Linden R, Hemmis C, Schmitt B, Ndoja A, Whitby F, Robinson H, et al. Structural basis for the activation and inhibition of the UCH37 deubiquitylase. Mol Cell. 2015;57:901-911 pubmed publisher
product information
Catalog Number :
61938
Product Name :
pETDUET1-hUCH37 (ISF2)-NFRKB(39-156)
article :
doi10.1016/j.molcel.2015.01.016
id9834
pubmed_id25702872
bacterial resistance :
Ampicillin
cloning :
backbonepETDUET1
backbone_mutationPreScission Protease site added between His-tag and MCS1
backbone_originNovagen
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth strain :
Co-expression of hUCH37 (ISF2) and hNFRKB (39-156) in E.coli
origin :
37
pi :
alt_names
UCHL5
cloning
clone_methodRestriction Enzyme
cloning_site_3NotI
cloning_site_5XhoI
promoterT7
sequencing_primer_3DuetDOWN1
sequencing_primer_5pET Upstream/T7
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesCGI-70, INO80R, UCH-L5, UCH37
geneUCHL5
id51377
genbank_ids
NP_001186190
mutationResidue E246 was deleted.
namehUCH37 (ISF2)
shRNA_sequence
size987
species
9606
Homo sapiens
tags
locationN terminal on backbone
tag6x HIS
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5NdeI
promoterT7
sequencing_primer_3T7 term
sequencing_primer_5DuetUP2
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesINO80G
geneNFRKB
id4798
genbank_ids
NP_001137307
mutationConstruct ends at residue 156, residues 2-38 deleted
namehNFRKB (39-156)
shRNA_sequence
size360
species
9606
Homo sapiens
tags
resistance markers :
2231
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA