This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pVITRO1-Trastuzumab-IgG1/
catalog :
61883
citations: 11
Reference
Yu B, Shi Q, Belk J, Yost K, Parker K, Li R, et al. Engineered cell entry links receptor biology with single-cell genomics. Cell. 2022;185:4904-4920.e22 pubmed publisher
Jaroentomeechai T, Kwon Y, Liu Y, Young O, Bhawal R, Wilson J, et al. A universal glycoenzyme biosynthesis pipeline that enables efficient cell-free remodeling of glycans. Nat Commun. 2022;13:6325 pubmed publisher
Zinngrebe J, Moepps B, Monecke T, Gierschik P, Schlichtig F, Barth T, et al. Compound heterozygous variants in OTULIN are associated with fulminant atypical late-onset ORAS. EMBO Mol Med. 2022;14:e14901 pubmed publisher
Cruz E, Sifniotis V, Sumer Bayraktar Z, Reslan M, Wilkinson White L, Cordwell S, et al. Glycan Profile Analysis of Engineered Trastuzumab with Rationally Added Glycosylation Sequons Presents Significantly Increased Glycan Complexity. Pharmaceutics. 2021;13: pubmed publisher
Natarajan A, Jaroentomeechai T, Cabrera Sánchez M, Mohammed J, Cox E, Young O, et al. Engineering orthogonal human O-linked glycoprotein biosynthesis in bacteria. Nat Chem Biol. 2020;16:1062-1070 pubmed publisher
Sun T, Kwok W, Chua K, Lo T, Potter J, Yew W, et al. Development of a Proline-Based Selection System for Reliable Genetic Engineering in Chinese Hamster Ovary Cells. ACS Synth Biol. 2020;9:1864-1872 pubmed publisher
Reslan M, Sifniotis V, Cruz E, Sumer Bayraktar Z, Cordwell S, Kayser V. Enhancing the stability of adalimumab by engineering additional glycosylation motifs. Int J Biol Macromol. 2020;158:189-196 pubmed publisher
Cox E, Thornlow D, Jones M, Fuller J, Merritt J, Paszek M, et al. Antibody-Mediated Endocytosis of Polysialic Acid Enables Intracellular Delivery and Cytotoxicity of a Glycan-Directed Antibody-Drug Conjugate. Cancer Res. 2019;79:1810-1821 pubmed publisher
Elgundi Z, Sifniotis V, Reslan M, Cruz E, Kayser V. Laboratory Scale Production and Purification of a Therapeutic Antibody. J Vis Exp. 2017;: pubmed publisher
Stengl A, Hörl D, Leonhardt H, Helma J. A Simple and Sensitive High-Content Assay for the Characterization of Antiproliferative Therapeutic Antibodies. SLAS Discov. 2017;22:309-315 pubmed publisher
Dodev T, Karagiannis P, Gilbert A, Josephs D, Bowen H, James L, et al. A tool kit for rapid cloning and expression of recombinant antibodies. Sci Rep. 2014;4:5885 pubmed publisher
product information
Catalog Number :
61883
Product Name :
pVITRO1-Trastuzumab-IgG1/
article :
doi10.1038/srep05885
id9039
pubmed_id25073855
bacterial resistance :
Hygromycin
cloning :
backbonepVITRO1-hygro-mcs
backbone_mutation
backbone_originInvivoGen
backbone_size6491
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Expresses HER2/neu receptor specific humanized IgG1/ antibody isotype (Trastuzumab-IgG1/ )
origin :
37
pi :
alt_names
Trastuzumab-IgG1
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promotermEF1 Prom
sequencing_primer_3AAAAAACCTCCCACACCTCC
sequencing_primer_5TTTTGAGCGGAGCTAATTCTCGGG
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameHER2/neu receptor specific humanized Gamma 1 heavy chain expression cassette
shRNA_sequence
size1410
species
9606
Homo sapiens
tags
alt_names
Trastuzumab-IgK
cloning
clone_methodLigation Independent Cloning
cloning_site_3
cloning_site_5
promoterrEF1 Prom
sequencing_primer_3TCTAGACCTGGAAAGACCAG
sequencing_primer_5GAGGCTAATTCTCAAGCCTC
site_3_destroyed
site_5_destroyed
entrez_gene
genbank_ids
mutation
nameHER2/neu receptor specific humanized kappa light chain expression cassette
shRNA_sequence
size702
species
9606
Homo sapiens
tags
resistance markers :
1785
tags :
Unknown
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA