(R42A) Cyclin B1-GFP (62) | Addgene 61849 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

(R42A) Cyclin B1-GFP (62)

catalog :

61849

citations: 5

Reference
Zeng Y, Yin L, Zhou J, Zeng R, Xiao Y, Black A, et al. MARK2 regulates chemotherapeutic responses through class IIa HDAC-YAP axis in pancreatic cancer. Oncogene. 2022;: pubmed publisher
Yin L, Zeng Y, Zeng R, Chen Y, Wang T, Rodabaugh K, et al. Protein kinase RNA-activated controls mitotic progression and determines paclitaxel chemosensitivity through B-cell lymphoma 2 in ovarian cancer. Oncogene. 2021;40:6772-6785 pubmed publisher
Zeng R, Wang Z, Li X, Chen Y, Yang S, Dong J. Cyclin-dependent kinase 1-mediated phosphorylation of protein kinase N1 promotes anchorage-independent growth and migration. Cell Signal. 2020;69:109546 pubmed publisher
Zhou J, Zhang L, Zhou W, Chen Y, Cheng Y, Dong J. LIMD1 phosphorylation in mitosis is required for mitotic progression and its tumor-suppressing activity. FEBS J. 2019;286:963-974 pubmed publisher
Clute P, Pines J. Temporal and spatial control of cyclin B1 destruction in metaphase. Nat Cell Biol. 1999;1:82-7 pubmed

product information

Catalog Number :

61849

Product Name :

(R42A) Cyclin B1-GFP (62)

article :

doi	10.1038/10049
id	9510
pubmed_id	10559878

bacterial resistance :

Ampicillin

cloning :

backbone

pCMX

backbone_mutation

backbone_origin

K.Umesono, Salk Institute

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

There is an addition conservative mutation at position Val5 (Ala in this construct).

growth strain :

mammalian expression of Cyclin B1 with R42A mutation in destruction box to make cyclin B1 non-destructable

origin :

pi :

alt_names

cloning

clone_method	Unknown
cloning_site_3
cloning_site_5
promoter	CMV
sequencing_primer_3
sequencing_primer_5	CMV-F
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	CCNB
gene	CCNB1
id	891

genbank_ids

mutation

V5A R42A

name

cyclin B1

shRNA_sequence

size

species

9606

Homo sapiens