This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
px330-gp78
catalog :
61833
citations: 1
Reference
Liu Y, Soetandyo N, Lee J, Liu L, Xu Y, Clemons W, et al. USP13 antagonizes gp78 to maintain functionality of a chaperone in ER-associated degradation. elife. 2014;3:e01369 pubmed publisher
product information
Catalog Number :
61833
Product Name :
px330-gp78
article :
doi10.7554/eLife.01369
id9482
pubmed_id24424410
bacterial resistance :
Ampicillin
cloning :
backbonepX330-U6-Chimeric_BB-CBh-hSpCas9
backbone_mutation
backbone_originAddgene
backbone_size8506
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
CRISPR
growth notes :
Because there is no selective marker, Western blot should be used to screen the knockout clones.
growth strain :
to make gp78 Knockout cell line
origin :
37
pi :
alt_names
AMFR, RNF45
cloning
clone_methodRestriction Enzyme
cloning_site_3BbsI
cloning_site_5BbsI
promoterU6
sequencing_primer_3aggcgggccatttaccgtaa
sequencing_primer_5CAAGGCTGTTAGAGAGATAATTGGA
site_3_destroyed1
site_5_destroyed1
entrez_gene
aliasesGP78, RNF45
geneAMFR
id267
genbank_ids
NM_001144.5
mutation
namegp78
shRNA_sequence
size20
species
9606
Homo sapiens
tags
resistance markers :
835
tags :
High Copy
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA