This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mCh-Rab7A
catalog :
61804
citations: 15
Reference
Talaia G, Bentley DeSousa A, Ferguson S. Lysosomal TBK1 Responds to Amino Acid Availability to Relieve Rab7-Dependent mTORC1 Inhibition. bioRxiv. 2023;: pubmed publisher
Hancock Cerutti W, Wu Z, Xu P, Yadavalli N, Leonzino M, Tharkeshwar A, et al. ER-lysosome lipid transfer protein VPS13C/PARK23 prevents aberrant mtDNA-dependent STING signaling. J Cell Biol. 2022;221: pubmed publisher
Trofimenko E, Homma Y, Fukuda M, Widmann C. The endocytic pathway taken by cationic substances requires Rab14 but not Rab5 and Rab7. Cell Rep. 2021;37:109945 pubmed publisher
Jiang X, Jia X, Sun J, Qi C, Lu L, Wang Y, et al. Overexpressed coiled-coil domain containing protein 8 (CCDC8) mediates newly synthesized HIV-1 Gag lysosomal degradation. Sci Rep. 2020;10:11416 pubmed publisher
Gonzales B, de Rocquigny H, Beziau A, Durand S, Burlaud Gaillard J, Lefebvre A, et al. Type I Phosphatidylinositol-4-Phosphate 5-Kinases α and γ Play a Key Role in Targeting HIV-1 Pr55Gag to the Plasma Membrane. J Virol. 2020;94: pubmed publisher
Khobrekar N, Quintremil S, Dantas T, Vallee R. The Dynein Adaptor RILP Controls Neuronal Autophagosome Biogenesis, Transport, and Clearance. Dev Cell. 2020;53:141-153.e4 pubmed publisher
Jongsma M, Bakker J, Cabukusta B, Liv N, van Elsland D, Fermie J, et al. SKIP-HOPS recruits TBC1D15 for a Rab7-to-Arl8b identity switch to control late endosome transport. EMBO J. 2020;39:e102301 pubmed publisher
Schwerk J, Soveg F, Ryan A, Thomas K, Hatfield L, Ozarkar S, et al. RNA-binding protein isoforms ZAP-S and ZAP-L have distinct antiviral and immune resolution functions. Nat Immunol. 2019;20:1610-1620 pubmed publisher
Wang G, Hu H, Chang Y, Huang Y, Song Z, Zhou S, et al. Rab7 regulates primary cilia disassembly through cilia excision. J Cell Biol. 2019;218:4030-4041 pubmed publisher
Lin A, Gill K, Castañeda M, Matucci I, Eder N, Claxton S, et al. Chemical genetic identification of GAK substrates reveals its role in regulating Na+/K+-ATPase. Life Sci Alliance. 2018;1:e201800118 pubmed publisher
Tábara L, Vicente J, Biazik J, Eskelinen E, Vincent O, Escalante R. Vacuole membrane protein 1 marks endoplasmic reticulum subdomains enriched in phospholipid synthesizing enzymes and is required for phosphoinositide distribution. Traffic. 2018;19:624-638 pubmed publisher
Wong Y, Ysselstein D, Krainc D. Mitochondria-lysosome contacts regulate mitochondrial fission via RAB7 GTP hydrolysis. Nature. 2018;554:382-386 pubmed publisher
Gowrishankar S, Wu Y, Ferguson S. Impaired JIP3-dependent axonal lysosome transport promotes amyloid plaque pathology. J Cell Biol. 2017;216:3291-3305 pubmed publisher
Tábara L, Escalante R. VMP1 Establishes ER-Microdomains that Regulate Membrane Contact Sites and Autophagy. PLoS ONE. 2016;11:e0166499 pubmed publisher
Rowland A, Chitwood P, Phillips M, Voeltz G. ER contact sites define the position and timing of endosome fission. Cell. 2014;159:1027-1041 pubmed publisher
product information
Catalog Number :
61804
Product Name :
mCh-Rab7A
article :
doi10.1016/j.cell.2014.10.023
id9490
pubmed_id25416943
bacterial resistance :
Kanamycin
cloning :
backbonepAcGFP1-C1
backbone_mutationmCh tag was amplified and inserted into the NheI and XhoI sites in order to replace the GFP.
backbone_originClontech
backbone_size4700
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
Human Rab7A fluorescently tagged with mCh on the N-terminus for mammalian expression
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5XhoI
promoterCMV
sequencing_primer_3none
sequencing_primer_5CCA TCG TGG AAC AGT ACG AACG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesCMT2B, PRO2706, RAB7
geneRAB7A
id7879
genbank_ids
NM_004637.5
mutationNone
nameRab7A
shRNA_sequence
size624
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagmCh
resistance markers :
1740
tags :
Unknown
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA