This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pCDNA-gp78-FLAG RING mutant C356G H361A
catalog :
61751
citations: 1
Reference
Liu Y, Soetandyo N, Lee J, Liu L, Xu Y, Clemons W, et al. USP13 antagonizes gp78 to maintain functionality of a chaperone in ER-associated degradation. elife. 2014;3:e01369 pubmed publisher
product information
Catalog Number :
61751
Product Name :
pCDNA-gp78-FLAG RING mutant C356G H361A
article :
doi10.7554/eLife.01369
id9482
pubmed_id24424410
bacterial resistance :
Ampicillin
cloning :
backbonepCDNA3
backbone_mutation
backbone_origin
backbone_size5400
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Mutations P179L, F262L, Y315C, and D346G found during Addgene's quality control process have no affect on plasmid function.
growth strain :
express C-terminally FLAG tagged human gp78
origin :
37
pi :
alt_names
AMFR, RNF45
cloning
clone_methodUnknown
cloning_site_3
cloning_site_5
promoterCMV
sequencing_primer_3ATTTAGGTGACACTATAG
sequencing_primer_5TAATACGACTCACTATAGGG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesGP78, RNF45
geneAMFR
id267
genbank_ids
NM_001144.5
mutationC356G H361A; P179L, F262L, Y315C, and D346G (please see depositor comment below)
namegp78
shRNA_sequence
size1932
species
9606
Homo sapiens
tags
locationC terminal on insert
tagFLAG
plasmid copy :
Wierds's Lab
resistance markers :
835
tags :
High Copy
terms :
Neomycin (select with G418)
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA