YFP-Parkin-IRES-zeo | Addgene 61728 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

YFP-Parkin-IRES-zeo

catalog :

61728

citations: 3

Reference
Gaschler M, Hu F, Feng H, Linkermann A, Min W, Stockwell B. Determination of the Subcellular Localization and Mechanism of Action of Ferrostatins in Suppressing Ferroptosis. ACS Chem Biol. 2018;13:1013-1020 pubmed publisher
Correia Melo C, Ichim G, Tait S, Passos J. Depletion of mitochondria in mammalian cells through enforced mitophagy. Nat Protoc. 2017;12:183-194 pubmed publisher
Tait S, Oberst A, Quarato G, Milasta S, Haller M, Wang R, et al. Widespread mitochondrial depletion via mitophagy does not compromise necroptosis. Cell Rep. 2013;5:878-85 pubmed publisher

product information

Catalog Number :

61728

Product Name :

YFP-Parkin-IRES-zeo

article :

doi	10.1016/j.celrep.2013.10.034
id	9473
pubmed_id	24268776

bacterial resistance :

Ampicillin

cloning :

backbone

LZRS

backbone_mutation

backbone_origin

backbone_size

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Mammalian Expression

growth notes :

Dr. Richard Youle provided YFP-Parkin (Addgene Plasmid 23955). Please note that the EBNA sequence may have ambiguous bases due to GC-rich regions.

growth strain :

Expression of human Parkin fused to eYFP

growth temp :

Slow growing. May need additional incubation time.

origin :

pi :

alt_names

Park 2

cloning

clone_method	Restriction Enzyme
cloning_site_3	EcoRI
cloning_site_5	BamHI
promoter
sequencing_primer_3	pCDH-Rev
sequencing_primer_5	EGFP-C
site_3_destroyed
site_5_destroyed

entrez_gene

aliases	AR-JP, LPRS2, PARK2, PDJ
gene	PRKN
id	5071

genbank_ids

NM_004562

mutation

name

Parkin

shRNA_sequence

size

species

9606

Homo sapiens