This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
mCherry-Parkin-IRES-zeo
catalog :
61727
citations: 3
Reference
Gaschler M, Hu F, Feng H, Linkermann A, Min W, Stockwell B. Determination of the Subcellular Localization and Mechanism of Action of Ferrostatins in Suppressing Ferroptosis. ACS Chem Biol. 2018;13:1013-1020 pubmed publisher
Correia Melo C, Ichim G, Tait S, Passos J. Depletion of mitochondria in mammalian cells through enforced mitophagy. Nat Protoc. 2017;12:183-194 pubmed publisher
Tait S, Oberst A, Quarato G, Milasta S, Haller M, Wang R, et al. Widespread mitochondrial depletion via mitophagy does not compromise necroptosis. Cell Rep. 2013;5:878-85 pubmed publisher
product information
Catalog Number :
61727
Product Name :
mCherry-Parkin-IRES-zeo
article :
doi10.1016/j.celrep.2013.10.034
id9473
pubmed_id24268776
bacterial resistance :
Ampicillin
cloning :
backboneLZRS
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth notes :
Dr. Richard Youle provided mCherry-Parkin (Addgene Plasmid 23956).
growth strain :
Expression of human Parkin fused to mCherry
growth temp :
Slow growing. May need additional incubation time.
origin :
30
pi :
alt_names
Park 2
cloning
clone_methodRestriction Enzyme
cloning_site_3EcoRI
cloning_site_5BamHI
promoter
sequencing_primer_3pCDH-Rev
sequencing_primer_5mCherry Forward
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAR-JP, LPRS2, PARK2, PDJ
genePRKN
id5071
genbank_ids
NM_004562
mutation
nameParkin
shRNA_sequence
size
species
9606
Homo sapiens
tags
locationN terminal on insert
tagmCherry
resistance markers :
1374
2195
tags :
Unknown
terms :
Zeocin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA