This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
p414GPD_Hsp82viiB
catalog :
61709
citations: 1
Reference
Mishra P, Bolon D. Designed Hsp90 heterodimers reveal an asymmetric ATPase-driven mechanism in vivo. Mol Cell. 2014;53:344-50 pubmed publisher
product information
Catalog Number :
61709
Product Name :
p414GPD_Hsp82viiB
article :
doi10.1016/j.molcel.2013.12.024
id9467
pubmed_id24462207
bacterial resistance :
Ampicillin
cloning :
backbonep414
backbone_mutation
backbone_origin
backbone_size
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Yeast Expression
growth notes :
*Note: This plasmid contains an A595C amino acid residue substitution. This residue change does not alter Hsp90 function in yeast but enables cross-dimer disulfide formation under oxidizing conditions. This construct accurately represents the plasmid as it was used in the associated publication.
growth strain :
Yeast plasmid with Hsp82 heterodimer construct.
origin :
37
pi :
alt_names
Hsp90
cloning
clone_methodGibson Cloning
cloning_site_3
cloning_site_5
promoterGPD1
sequencing_primer_3gtaaaacgacggccagtgagc
sequencing_primer_5cagctatgaccatgattacg
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesYPL240C, HSP90
geneHSP82
id855836
genbank_ids
mutationHeteromeric coiled coil inserted after amino acid 678; A595C*
nameHsp82viiB
shRNA_sequence
size2000
species
4932
Saccharomyces cerevisiae
tags
locationN terminal on insert
tag6xHis
resistance markers :
1400
tags :
High Copy
terms :
TRP1
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA