pETDuet-1 Hepatitis C Virus NS3/NS4A | Addgene 61696 product information

This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.

product summary

company name :

Addgene

product type :

cDNA

product name :

pETDuet-1 Hepatitis C Virus NS3/NS4A

catalog :

61696

citations: 2

Reference
Xie S, Zhu C, Yang L, Li H, Zhu H, Nie Z, et al. Programmable Proteolysis-Activated Transcription for Highly Sensitive Ratiometric Electrochemical Detection of Viral Protease. Anal Chem. 2023;95:10728-10735 pubmed publisher
Lee H, Zhu T, Patel K, Zhang Y, Truong L, Hevener K, et al. High-throughput screening (HTS) and hit validation to identify small molecule inhibitors with activity against NS3/4A proteases from multiple hepatitis C virus genotypes. PLoS ONE. 2013;8:e75144 pubmed publisher

product information

Catalog Number :

61696

Product Name :

pETDuet-1 Hepatitis C Virus NS3/NS4A

article :

doi	PONE-D-13-22581 [pii]
id	9460
pubmed_id	24130685

bacterial resistance :

Ampicillin

cloning :

backbone

pETDuet-1

backbone_mutation

backbone_origin

EMD

backbone_size

5420

promoter

sequencing_primer_3

sequencing_primer_5

vector_types

Bacterial Expression

growth notes :

For protein expression, grow in Rosetta cells. 25 degree induction for 14-15 hours with 0.5 mM IPTG

growth strain :

inducible dual expression of NS3 protease along with its cofactor NS4A

origin :

pi :

alt_names

cloning

clone_method	Restriction Enzyme
cloning_site_3	HindIII
cloning_site_5	NcoI
promoter	T7
sequencing_primer_3
sequencing_primer_5	pBRrevBam
site_3_destroyed
site_5_destroyed

entrez_gene

genbank_ids

mutation

name

NS4A

shRNA_sequence

size

species

32644

Other

Hepatitis C Virus