This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pLJM5-I560S-mDPYD
catalog :
61615
citations: 1
Reference
Shaul Y, Freinkman E, Comb W, Cantor J, Tam W, Thiru P, et al. Dihydropyrimidine accumulation is required for the epithelial-mesenchymal transition. Cell. 2014;158:1094-1109 pubmed publisher
product information
Catalog Number :
61615
Product Name :
pLJM5-I560S-mDPYD
article :
doi10.1016/j.cell.2014.07.032
id9312
pubmed_id25171410
bacterial resistance :
Ampicillin
cloning :
backbonepLJM5
backbone_mutation
backbone_origin
backbone_size8001
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Mammalian Expression
growth strain :
The mouse Dpyd was used to rescue the human DPYD hairpin affect on EMT
origin :
37
pi :
alt_names
Dpyd
cloning
clone_methodRestriction Enzyme
cloning_site_3KpnI
cloning_site_5NcoI
promoterCMV
sequencing_primer_3CCCTTTTCTTTTAAAATTGTGGATGAATACTGCC
sequencing_primer_5TGTACGGTGGGAGGTCTATATAAG
site_3_destroyed
site_5_destroyed
entrez_gene
aliasesAI315208, DPD, E330028L06Rik
geneDpyd
id99586
genbank_ids
mutationChanged Isoleucine at position 560 to Serine
nameDihydropyrimidine dehydrogenase
shRNA_sequence
size3086
species
10090
Mus musculus
tags
plasmid copy :
Thermo Scientific Biochem Sciences (Catalog Number: 4235971)
resistance markers :
432
tags :
High Copy
terms :
Hygromycin
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA