This webpage contains legacy information. The product is either no longer available from the supplier or has been delisted at Labome.
product summary
company name :
Addgene
product type :
cDNA
product name :
pOPINS-USP21 (USP, aa 196-565)
catalog :
61585
citations: 2
Reference
Pedrosa A, Francisco T, Rodrigues T, Ferreira M, van der Heden van Noort G, Azevedo J. The Extraction Mechanism of Monoubiquitinated PEX5 from the Peroxisomal Membrane. J Mol Biol. 2023;435:167896 pubmed publisher
Ye Y, Akutsu M, Reyes Turcu F, Enchev R, Wilkinson K, Komander D. Polyubiquitin binding and cross-reactivity in the USP domain deubiquitinase USP21. EMBO Rep. 2011;12:350-7 pubmed publisher
product information
Catalog Number :
61585
Product Name :
pOPINS-USP21 (USP, aa 196-565)
article :
doi10.1038/embor.2011.17
id9414
pubmed_id21399617
bacterial resistance :
Kanamycin
cloning :
backbonepOPINS
backbone_mutation
backbone_originOPPF-UK
backbone_size5906
promoter
sequencing_primer_3
sequencing_primer_5
vector_types
Bacterial Expression
growth notes :
Mevissen et al Cell. 2013 Jul 3;154(1):169-84. doi: 10.1016/j.cell.2013.05.046. Hospenthal MK, Mevissen TET, Komander D. Nature Protoc. 2015 Jan 29;10:349-361. doi:10.1038/nprot.2015.018
growth strain :
Expresses human USP21 (USP domain) in E. coli.
origin :
37
pi :
alt_names
cloning
clone_methodRestriction Enzyme
cloning_site_3HindIII
cloning_site_5KpnI
promoterT7
sequencing_primer_3T7 Terminal
sequencing_primer_5CGGAAGACCTGGATATGGAAGAC
site_3_destroyed
site_5_destroyed1
entrez_gene
aliasesUSP16, USP23
geneUSP21
id27005
genbank_ids
NM_012475.4
mutationDeleted aa 1-195.
nameUSP21 (Ubiquitin carboxyl-terminal hydrolase 21)
shRNA_sequence
size1110
species
9606
Homo sapiens
tags
locationN terminal on backbone
tagHis6-SUMO
resistance markers :
2189
tags :
Unknown
company information
Addgene
490 Arsenal Way, Suite 100
Watertown, MA 02472
info@addgene.org
https://www.addgene.org
617.225.9000
headquarters: USA